An Efficient Procedure for Marker-Free Mutagenesis of S. coelicolor by Site-Specific Recombination for Secondary Metabolite Overproduction

• Bo Zhang ,

  Contributed equally to this work with: Bo Zhang, Lin Zhang

  Affiliation Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China

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• Lin Zhang ,

  Contributed equally to this work with: Bo Zhang, Lin Zhang

  Affiliations Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China, Key Laboratory of Synthetic biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

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• Ruixue Dai,

  Affiliation Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China

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• Meiying Yu,

  Affiliation Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China

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• Guoping Zhao ,

  * E-mail: gpzhao@sibs.ac.cn (GZ); xmding74@fudan.edu.cn (XD)

  Affiliations Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China, Key Laboratory of Synthetic biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China, Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China, Department of Microbiology and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China

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• Xiaoming Ding

  * E-mail: gpzhao@sibs.ac.cn (GZ); xmding74@fudan.edu.cn (XD)

  Affiliation Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China

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