Feeder-Free Generation and Long-Term Culture of Human Induced Pluripotent Stem Cells Using Pericellular Matrix of Decidua Derived Mesenchymal Cells

Hayato Fukusumi; Tomoko Shofuda; Daisuke Kanematsu; Atsuyo Yamamoto; Hiroshi Suemizu; Masato Nakamura; Mami Yamasaki; Masatoshi Ohgushi; Yoshiki Sasai; Yonehiro Kanemura

doi:10.1371/journal.pone.0055226

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Open Access

Peer-reviewed

Research Article

Feeder-Free Generation and Long-Term Culture of Human Induced Pluripotent Stem Cells Using Pericellular Matrix of Decidua Derived Mesenchymal Cells

Hayato Fukusumi,

Affiliation Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan
⨯
Tomoko Shofuda,

Affiliation Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan
⨯
Daisuke Kanematsu,

Affiliation Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan
⨯
Atsuyo Yamamoto,

Affiliation Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan
⨯
Hiroshi Suemizu,

Affiliation Biomedical Research Department, Central Institute for Experimental Animals, Kawasaki, Kanagawa, Japan
⨯
Masato Nakamura,

Affiliations Biomedical Research Department, Central Institute for Experimental Animals, Kawasaki, Kanagawa, Japan, Department of Pathology and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯
Mami Yamasaki,

Affiliations Division of Molecular Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan, Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan, Department of Pediatric Neurosurgery, Takatsuki General Hospital, Takatsuki, Osaka, Japan
⨯
Masatoshi Ohgushi,

Affiliation Unit for Human Stem Cell Technology, Center for Developmental Biology, RIKEN, Kobe, Hyogo, Japan
⨯
Yoshiki Sasai,

Affiliation Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe, Hyogo, Japan
⨯
Yonehiro Kanemura

* E-mail: kanemura@onh.go.jp

Affiliations Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan, Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan
⨯

Feeder-Free Generation and Long-Term Culture of Human Induced Pluripotent Stem Cells Using Pericellular Matrix of Decidua Derived Mesenchymal Cells

Hayato Fukusumi,
Tomoko Shofuda,
Daisuke Kanematsu,
Atsuyo Yamamoto,
Hiroshi Suemizu,
Masato Nakamura,
Mami Yamasaki,
Masatoshi Ohgushi,
Yoshiki Sasai,
Yonehiro Kanemura

Published: January 31, 2013
https://doi.org/10.1371/journal.pone.0055226

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