Detection of Low-Abundance KRAS Mutations in Colorectal Cancer Using Microfluidic Capillary Electrophoresis-Based Restriction Fragment Length Polymorphism Method with Optimized Assay Conditions

Open Access

Peer-reviewed

Research Article

Huidan Zhang ,

Contributed equally to this work with: Huidan Zhang, Jin Song

Affiliation Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, China
⨯
Jin Song ,

Contributed equally to this work with: Huidan Zhang, Jin Song

Affiliation Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, China
⨯
Hui Ren,

Affiliation Department of Colon Surgery, Shenyang Anal Rectum Hospital, Shenyang, China
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Zhangrun Xu,

Affiliation Research Center for Analytical Sciences, Northeastern University, Shenyang, China
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Xiaonan Wang,

Affiliation Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, China
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Lianfeng Shan,

Affiliation Department of Mathematics, College of Basic Medical Sciences, China Medical University, Shenyang, China
⨯
Jin Fang

* E-mail: jfang61@gmail.com or jfang61@netease.com

Affiliation Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, China
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Restriction fragment length polymorphism analysis
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Mutation detection
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Colorectal cancer
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Direct sequencing
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Point mutation
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Polymerase chain reaction
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Microfluidics
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HT29 cells
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