Standard Addition Quantitative Real-Time PCR (SAQPCR): A Novel Approach for Determination of Transgene Copy Number Avoiding PCR Efficiency Estimation

Yuji Huang; Xueren Yin; Changqing Zhu; Weiwei Wang; Donald Grierson; Changjie Xu; Kunsong Chen

doi:10.1371/journal.pone.0053489

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Peer-reviewed

Research Article

Standard Addition Quantitative Real-Time PCR (SAQPCR): A Novel Approach for Determination of Transgene Copy Number Avoiding PCR Efficiency Estimation

Yuji Huang ,

Contributed equally to this work with: Yuji Huang, Xueren Yin

Affiliation Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, P. R. China
⨯
Xueren Yin ,

Contributed equally to this work with: Yuji Huang, Xueren Yin

Affiliation Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, P. R. China
⨯
Changqing Zhu,

Affiliation Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, P. R. China
⨯
Weiwei Wang,

Affiliation Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, P. R. China
⨯
Donald Grierson,

Affiliations Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, P. R. China, Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom
⨯
Changjie Xu ,

* E-mail: chjxu@zju.edu.cn

Affiliation Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, P. R. China
⨯
Kunsong Chen

Affiliation Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, P. R. China
⨯

Standard Addition Quantitative Real-Time PCR (SAQPCR): A Novel Approach for Determination of Transgene Copy Number Avoiding PCR Efficiency Estimation

Yuji Huang,
Xueren Yin,
Changqing Zhu,
Weiwei Wang,
Donald Grierson,
Changjie Xu,
Kunsong Chen

Published: January 7, 2013
https://doi.org/10.1371/journal.pone.0053489

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