Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints

Open Access

Peer-reviewed

Research Article

Alexander Ulrich,

Affiliations Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany
⨯
Kasper R. Andersen,

Affiliation Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
⨯
Thomas U. Schwartz

* E-mail: tus@mit.edu

Affiliation Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
⨯

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Cloning
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Polymerase chain reaction
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Plasmid construction
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DNA cloning
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Gel electrophoresis
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DNA polymerase
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Homologous recombination
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Ligases
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