Purification and Characterisation of Immunoglobulins from the Australian Black Flying Fox (Pteropus alecto) Using Anti-Fab Affinity Chromatography Reveals the Low Abundance of IgA

James W. Wynne; Antonio Di Rubbo; Brian J. Shiell; Gary Beddome; Christopher Cowled; Grantley R. Peck; Jing Huang; Samantha L. Grimley; Michelle L. Baker; Wojtek P. Michalski

doi:10.1371/journal.pone.0052930

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Open Access

Peer-reviewed

Research Article

Purification and Characterisation of Immunoglobulins from the Australian Black Flying Fox (Pteropus alecto) Using Anti-Fab Affinity Chromatography Reveals the Low Abundance of IgA

James W. Wynne ,

Contributed equally to this work with: James W. Wynne, Antonio Di Rubbo

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯
Antonio Di Rubbo ,

Contributed equally to this work with: James W. Wynne, Antonio Di Rubbo

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯
Brian J. Shiell,

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯
Gary Beddome,

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯
Christopher Cowled,

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯
Grantley R. Peck,

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯
Jing Huang,

Affiliations Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia, School of Life Science, East China Normal University, Shanghai, China
⨯
Samantha L. Grimley,

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯
Michelle L. Baker,

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯
Wojtek P. Michalski

* E-mail: wojtek.michalski@csiro.au

Affiliation Australian Animal Health Laboratory, CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia
⨯

Purification and Characterisation of Immunoglobulins from the Australian Black Flying Fox (Pteropus alecto) Using Anti-Fab Affinity Chromatography Reveals the Low Abundance of IgA

James W. Wynne,
Antonio Di Rubbo,
Brian J. Shiell,
Gary Beddome,
Christopher Cowled,
Grantley R. Peck,
Jing Huang,
Samantha L. Grimley,
Michelle L. Baker,
Wojtek P. Michalski

Published: January 7, 2013
https://doi.org/10.1371/journal.pone.0052930

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