Comparison Study of MS-HRM and Pyrosequencing Techniques for Quantification of APC and CDKN2A Gene Methylation

Francesca Migheli; Andrea Stoccoro; Fabio Coppedè; Wan Adnan Wan Omar; Alessandra Failli; Rita Consolini; Massimo Seccia; Roberto Spisni; Paolo Miccoli; John C. Mathers; Lucia Migliore

doi:10.1371/journal.pone.0052501

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Open Access

Peer-reviewed

Research Article

Comparison Study of MS-HRM and Pyrosequencing Techniques for Quantification of APC and CDKN2A Gene Methylation

Francesca Migheli,

Affiliations Department of Translational Research and New Technologies in Medicine and Surgery, Division of Medical Genetics, University of Pisa, Pisa, Italy, Istituto Toscano Tumori (ITT), Florence, Italy
⨯
Andrea Stoccoro,

Affiliation Department of Translational Research and New Technologies in Medicine and Surgery, Division of Medical Genetics, University of Pisa, Pisa, Italy
⨯
Fabio Coppedè,

Affiliations Department of Laboratory Medicine, Pisa University Hospital (AOUP), Pisa, Italy, Istituto Toscano Tumori (ITT), Florence, Italy
⨯
Wan Adnan Wan Omar,

Affiliations Advance Medical and Dental Institute, University Sains Malaysia, Penang, Malaysia, Human Nutrition Research Centre, Institute for Ageing & Health, Biomedical Research Building, Campus for Ageing & Vitality, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Alessandra Failli,

Affiliation Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
⨯
Rita Consolini,

Affiliation Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
⨯
Massimo Seccia,

Affiliation Department of Surgery, Medical, Molecular, and Critical Area Pathology, University of Pisa, Pisa, Italy
⨯
Roberto Spisni,

Affiliation Department of Surgery, Medical, Molecular, and Critical Area Pathology, University of Pisa, Pisa, Italy
⨯
Paolo Miccoli,

Affiliation Department of Surgery, Medical, Molecular, and Critical Area Pathology, University of Pisa, Pisa, Italy
⨯
John C. Mathers,

Affiliation Human Nutrition Research Centre, Institute for Ageing & Health, Biomedical Research Building, Campus for Ageing & Vitality, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Lucia Migliore

* E-mail: l.migliore@geog.unipi.it

Affiliations Department of Translational Research and New Technologies in Medicine and Surgery, Division of Medical Genetics, University of Pisa, Pisa, Italy, Department of Laboratory Medicine, Pisa University Hospital (AOUP), Pisa, Italy, Istituto Toscano Tumori (ITT), Florence, Italy
⨯

Comparison Study of MS-HRM and Pyrosequencing Techniques for Quantification of APC and CDKN2A Gene Methylation

Francesca Migheli,
Andrea Stoccoro,
Fabio Coppedè,
Wan Adnan Wan Omar,
Alessandra Failli,
Rita Consolini,
Massimo Seccia,
Roberto Spisni,
Paolo Miccoli,
John C. Mathers

Published: January 11, 2013
https://doi.org/10.1371/journal.pone.0052501

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DNA methylation
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Antigen-presenting cells
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Melting
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Methylation
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Interpolation
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Tumor suppressor genes
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Polymerase chain reaction
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Cancers and neoplasms
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