Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

David J. Leibly; Trang Nhu Nguyen; Louis T. Kao; Stephen N. Hewitt; Lynn K. Barrett; Wesley C. Van Voorhis

doi:10.1371/journal.pone.0052482

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Peer-reviewed

Research Article

Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

David J. Leibly,

Affiliations Department of Allergy and Infectious Disease, School of Medicine, University of Washington, Seattle, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, Washington, United States of America
⨯
Trang Nhu Nguyen,

Affiliations Department of Allergy and Infectious Disease, School of Medicine, University of Washington, Seattle, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, Washington, United States of America
⨯
Louis T. Kao,

Affiliations Department of Allergy and Infectious Disease, School of Medicine, University of Washington, Seattle, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, Washington, United States of America
⨯
Stephen N. Hewitt,

Affiliations Department of Allergy and Infectious Disease, School of Medicine, University of Washington, Seattle, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, Washington, United States of America
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Lynn K. Barrett,

Affiliations Department of Allergy and Infectious Disease, School of Medicine, University of Washington, Seattle, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, Washington, United States of America
⨯
Wesley C. Van Voorhis

* E-mail: wesley@uw.edu

Affiliations Department of Allergy and Infectious Disease, School of Medicine, University of Washington, Seattle, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, Washington, United States of America
⨯

Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

David J. Leibly,
Trang Nhu Nguyen,
Louis T. Kao,
Stephen N. Hewitt,
Lynn K. Barrett,
Wesley C. Van Voorhis

Published: December 20, 2012
https://doi.org/10.1371/journal.pone.0052482

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Solubility
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Trehalose
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Recombinant proteins
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Protein folding
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Toxoplasma gondii
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Protein expression
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Glycine
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Mannitol
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