A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

Tatsuhiko Ozawa; Xiuhong Piao; Eiji Kobayashi; Yue Zhou; Hiroaki Sakurai; Tsugunobu Andoh; Aishun Jin; Hiroyuki Kishi; Atsushi Muraguchi

doi:10.1371/journal.pone.0052383

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Peer-reviewed

Research Article

A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

Tatsuhiko Ozawa ,

Contributed equally to this work with: Tatsuhiko Ozawa, Xiuhong Piao

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
⨯
Xiuhong Piao ,

Contributed equally to this work with: Tatsuhiko Ozawa, Xiuhong Piao

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
⨯
Eiji Kobayashi,

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
⨯
Yue Zhou,

Affiliation Department of Cancer Cell Biology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
⨯
Hiroaki Sakurai,

Affiliation Department of Cancer Cell Biology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
⨯
Tsugunobu Andoh,

Affiliation Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
⨯
Aishun Jin,

Affiliations Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan, Department of Immunology, College of Basic Medical Sciences, Harbin Medical University, Nangang District, Harbin, China
⨯
Hiroyuki Kishi ,

* E-mail: immkishi@med.u-toyama.ac.jp

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
⨯
Atsushi Muraguchi

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
⨯

A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

Tatsuhiko Ozawa,
Xiuhong Piao,
Eiji Kobayashi,
Yue Zhou,
Hiroaki Sakurai,
Tsugunobu Andoh,
Aishun Jin,
Hiroyuki Kishi,
Atsushi Muraguchi

Published: December 26, 2012
https://doi.org/10.1371/journal.pone.0052383

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