Generation of a Genome Scale Lentiviral Vector Library for EF1α Promoter-Driven Expression of Human ORFs and Identification of Human Genes Affecting Viral Titer

Open Access

Peer-reviewed

Research Article

Dubravka Škalamera ,

* E-mail: d.skalamera@uq.edu.au

Affiliation University of Queensland Diamantina Institute, Princess Alexandra Hospital, Brisbane, Queensland, Australia
⨯
Mareike Dahmer,

Affiliation University of Queensland Diamantina Institute, Princess Alexandra Hospital, Brisbane, Queensland, Australia
⨯
Amy S. Purdon,

Current address: Mater Medical Research Institute, Brisbane, Queensland, Australia

Affiliation University of Queensland Diamantina Institute, Princess Alexandra Hospital, Brisbane, Queensland, Australia
⨯
Benjamin M. Wilson,

Affiliation University of Queensland Diamantina Institute, Princess Alexandra Hospital, Brisbane, Queensland, Australia
⨯
Max V. Ranall,

Affiliation University of Queensland Diamantina Institute, Princess Alexandra Hospital, Brisbane, Queensland, Australia
⨯
Antje Blumenthal,

Affiliation University of Queensland Diamantina Institute, Princess Alexandra Hospital, Brisbane, Queensland, Australia
⨯
Brian Gabrielli,

Affiliation University of Queensland Diamantina Institute, Princess Alexandra Hospital, Brisbane, Queensland, Australia
⨯
Thomas J. Gonda

Current address: School of Pharmacy, University of Queensland, Pharmacy Australia Centre of Excellence, Brisbane, Queensland, Australia

Affiliation University of Queensland Diamantina Institute, Princess Alexandra Hospital, Brisbane, Queensland, Australia
⨯

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Transfection
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Cloning
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Library screening
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DNA cloning
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Plasmid construction
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Viral vectors
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Gene prediction
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Genomic library screening
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