Fungal His-Tagged Nitrilase from Gibberella intermedia: Gene Cloning, Heterologous Expression and Biochemical Properties

Open Access

Peer-reviewed

Research Article

Jin-Song Gong,

Affiliations Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, People's Republic of China, The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, People's Republic of China
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Heng Li,

Affiliation Laboratory of Bioactive Products Processing Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, People's Republic of China
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Xiao-Yan Zhu,

Affiliation The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, People's Republic of China
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Zhen-Ming Lu,

Affiliation Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, People's Republic of China
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Yan Wu,

Affiliation The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, People's Republic of China
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Jing-Song Shi,

Affiliation Laboratory of Bioactive Products Processing Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, People's Republic of China
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Zheng-Hong Xu

* E-mail: zhenghxu@jiangnan.edu.cn

Affiliations Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, People's Republic of China, The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, People's Republic of China
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Fungal genetics
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Nitriles
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Polymerase chain reaction
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Carboxylic acids
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Cloning
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Gene cloning
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Ammonia
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Recombination reactions
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