High Osmolality Vitrification: A New Method for the Simple and Temperature-Permissive Cryopreservation of Mouse Embryos

• Keiji Mochida,

  Affiliation RIKEN BioResource Center, Tsukuba, Ibaraki, Japan

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• Ayumi Hasegawa,

  Affiliation RIKEN BioResource Center, Tsukuba, Ibaraki, Japan

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• Ming-Wen Li,

  Affiliation Mouse Biology Program, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America

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• Martin D. Fray,

  Affiliation Medical Research Council Mary Lyon Centre, Harwell Science and Innovation Centre, Oxon, United Kingdom

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• Seiji Kito,

  Affiliation National Institute of Radiological Sciences, Chiba-shi, Chiba, Japan

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• Jadine M. Vallelunga,

  Affiliation Mouse Biology Program, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America

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• K. C. Kent Lloyd,

  Affiliation Mouse Biology Program, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America

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• Atsushi Yoshiki,

  Affiliation RIKEN BioResource Center, Tsukuba, Ibaraki, Japan

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• Yuichi Obata,

  Affiliation RIKEN BioResource Center, Tsukuba, Ibaraki, Japan

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• Atsuo Ogura

  * E-mail: ogura@rtc.riken.go.jp

  Affiliations RIKEN BioResource Center, Tsukuba, Ibaraki, Japan, Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki, Japan, The Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan

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