Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores

Romain Laine; Daniel W. Stuckey; Hugh Manning; Sean C. Warren; Gordon Kennedy; David Carling; Chris Dunsby; Alessandro Sardini; Paul M. W. French

doi:10.1371/journal.pone.0049200

Loading metrics

Open Access

Peer-reviewed

Research Article

Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores

Romain Laine ,

* E-mail: romain.laine06@imperial.ac.uk

Affiliations Institute of Chemical Biology (ICB), Imperial College of Science, Technology & Medicine, Institute of Chemical Biology (ICB), London, England, Photonics Group, Blackett Lab, Imperial College of Science, Technology & Medicine, London, England, Medical Research Council (MRC) Clinical Sciences Centre, Imperial College of Science, Technology & Medicine, London, England
⨯
Daniel W. Stuckey,

Current address: Molecular Neurotherapy and Imaging Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America

Affiliation Medical Research Council (MRC) Clinical Sciences Centre, Imperial College of Science, Technology & Medicine, London, England
⨯
Hugh Manning,

Affiliation Photonics Group, Blackett Lab, Imperial College of Science, Technology & Medicine, London, England
⨯
Sean C. Warren,

Affiliation Photonics Group, Blackett Lab, Imperial College of Science, Technology & Medicine, London, England
⨯
Gordon Kennedy,

Affiliation Photonics Group, Blackett Lab, Imperial College of Science, Technology & Medicine, London, England
⨯
David Carling,

Affiliation Medical Research Council (MRC) Clinical Sciences Centre, Imperial College of Science, Technology & Medicine, London, England
⨯
Chris Dunsby ,

Contributed equally to this work with: Chris Dunsby, Alessandro Sardini, Paul M. W. French

Affiliation Photonics Group, Blackett Lab, Imperial College of Science, Technology & Medicine, London, England
⨯
Alessandro Sardini ,

Contributed equally to this work with: Chris Dunsby, Alessandro Sardini, Paul M. W. French

Affiliation Medical Research Council (MRC) Clinical Sciences Centre, Imperial College of Science, Technology & Medicine, London, England
⨯
Paul M. W. French

Contributed equally to this work with: Chris Dunsby, Alessandro Sardini, Paul M. W. French

Affiliation Photonics Group, Blackett Lab, Imperial College of Science, Technology & Medicine, London, England
⨯

Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores

Romain Laine,
Daniel W. Stuckey,
Hugh Manning,
Sean C. Warren,
Gordon Kennedy,
David Carling,
Chris Dunsby,
Alessandro Sardini,
Paul M. W. French

Published: November 9, 2012
https://doi.org/10.1371/journal.pone.0049200

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Fluorescence resonance energy transfer
Is the Subject Area "Fluorescence resonance energy transfer" applicable to this article?

Thanks for your feedback.
Fluorescence
Is the Subject Area "Fluorescence" applicable to this article?

Thanks for your feedback.
Biosensors
Is the Subject Area "Biosensors" applicable to this article?

Thanks for your feedback.
Cytosol
Is the Subject Area "Cytosol" applicable to this article?

Thanks for your feedback.
Troponin
Is the Subject Area "Troponin" applicable to this article?

Thanks for your feedback.
Fluorescence imaging
Is the Subject Area "Fluorescence imaging" applicable to this article?

Thanks for your feedback.
Photons
Is the Subject Area "Photons" applicable to this article?

Thanks for your feedback.
Calcium imaging
Is the Subject Area "Calcium imaging" applicable to this article?

Thanks for your feedback.