Towards Optimising the Production of and Expression from Polycistronic Vectors in Embryonic Stem Cells

Open Access

Peer-reviewed

Research Article

Steven Y. Gao,

Affiliation Centre for Developmental and Regenerative Medicine, Kolling Institute for Medical Research, Sydney Medical School, University of Sydney, Sydney, NSW, Australia
⨯
Michelle M. Jack,

Affiliations Centre for Developmental and Regenerative Medicine, Kolling Institute for Medical Research, Sydney Medical School, University of Sydney, Sydney, NSW, Australia, Department of Endocrinology, Royal North Shore Hospital, Sydney, NSW, Australia
⨯
Christopher O’Neill

* E-mail: chris.oneill@sydney.edu.au

Affiliation Centre for Developmental and Regenerative Medicine, Kolling Institute for Medical Research, Sydney Medical School, University of Sydney, Sydney, NSW, Australia
⨯

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Transfection
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Polymerase chain reaction
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Plasmid construction
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Embryonic stem cells
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Protein expression
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Gene expression
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Restriction enzyme digestion
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Vector construction
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