Site Saturation Mutagenesis Demonstrates a Central Role for Cysteine 298 as Proton Donor to the Catalytic Site in CaHydA [FeFe]-Hydrogenase

Open Access

Peer-reviewed

Research Article

Simone Morra,

Affiliation Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
⨯
Alberto Giraudo,

Affiliation Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
⨯
Giovanna Di Nardo,

Affiliation Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
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Paul W. King,

Affiliation Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado, United States of America
⨯
Gianfranco Gilardi,

Affiliation Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
⨯
Francesca Valetti

* E-mail: francesca.valetti@unito.it

Affiliation Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
⨯

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Cysteine
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Hydrogen
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Protons
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Aspartic acid
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Cloning
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Library screening
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Catalysis
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Molecular evolution
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