Application of a Novel Strategy of Engineering Conditional Alleles to a Single Exon Gene, Sox2

Nikolaos Mandalos; Marannia Saridaki; Jessica Lea Harper; Anastasia Kotsoni; Peter Yang; Aris N. Economides; Eumorphia Remboutsika

doi:10.1371/journal.pone.0045768

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Peer-reviewed

Research Article

Application of a Novel Strategy of Engineering Conditional Alleles to a Single Exon Gene, Sox2

Nikolaos Mandalos,

Affiliation Stem Cell Biology Laboratory, Institute of Molecular Biology and Genetics, Biomedical Sciences Research Centre “Alexander Fleming”, Vari, Greece
⨯
Marannia Saridaki,

Current address: Biomedical Research Foundation of the Academy of Athens, Athens, Greece

Affiliation Stem Cell Biology Laboratory, Institute of Molecular Biology and Genetics, Biomedical Sciences Research Centre “Alexander Fleming”, Vari, Greece
⨯
Jessica Lea Harper,

Affiliations Stem Cell Biology Laboratory, Institute of Molecular Biology and Genetics, Biomedical Sciences Research Centre “Alexander Fleming”, Vari, Greece, Regeneron Pharmaceuticals Inc., Tarrytown, New York, United States of America
⨯
Anastasia Kotsoni,

Current address: Institute of Immunology, Biomedical Sciences Research Centre “Alexander Fleming”, Vari, Greece

Affiliation Stem Cell Biology Laboratory, Institute of Molecular Biology and Genetics, Biomedical Sciences Research Centre “Alexander Fleming”, Vari, Greece
⨯
Peter Yang,

Affiliation Regeneron Pharmaceuticals Inc., Tarrytown, New York, United States of America
⨯
Aris N. Economides ,

* E-mail: remboutsika@gmail.com (ER); aris.economides@regeneron.com (ANE)

Affiliation Regeneron Pharmaceuticals Inc., Tarrytown, New York, United States of America
⨯
Eumorphia Remboutsika

* E-mail: remboutsika@gmail.com (ER); aris.economides@regeneron.com (ANE)

Affiliation Stem Cell Biology Laboratory, Institute of Molecular Biology and Genetics, Biomedical Sciences Research Centre “Alexander Fleming”, Vari, Greece
⨯

Application of a Novel Strategy of Engineering Conditional Alleles to a Single Exon Gene, Sox2

Nikolaos Mandalos,
Marannia Saridaki,
Jessica Lea Harper,
Anastasia Kotsoni,
Peter Yang,
Aris N. Economides,
Eumorphia Remboutsika

Published: September 27, 2012
https://doi.org/10.1371/journal.pone.0045768

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Alleles
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Introns
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Embryos
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Polymerase chain reaction
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Genetic loci
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DNA methylation
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Gene targeting
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Genotyping
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