An Improved Protocol for Sequencing of Repetitive Genomic Regions and Structural Variations Using Mutagenesis and Next Generation Sequencing

Open Access

Peer-reviewed

Research Article

Botond Sipos ,

* E-mail: sbotond@ebi.ac.uk

Affiliation European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
⨯
Tim Massingham,

Affiliation European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
⨯
Adrian M. Stütz,

Affiliation European Molecular Biology Laboratory (EMBL), Genome Biology Research Unit, Heidelberg, Germany
⨯
Nick Goldman

Affiliation European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
⨯

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Polymerase chain reaction
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Genomics
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Sequence alignment
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Substitution mutation
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Genome sequencing
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Next-generation sequencing
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Mutagenesis
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Molecular cloning
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