Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition

Open Access

Peer-reviewed

Research Article

Stavroula K. Hatzios,

Affiliations Division of Infectious Diseases, Brigham and Women’s Hospital, Boston, Massachusetts, United States of America, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America, Howard Hughes Medical Institute, Boston, Massachusetts, United States of America
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Simon Ringgaard,

Affiliations Division of Infectious Diseases, Brigham and Women’s Hospital, Boston, Massachusetts, United States of America, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America, Howard Hughes Medical Institute, Boston, Massachusetts, United States of America
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Brigid M. Davis,

Affiliations Division of Infectious Diseases, Brigham and Women’s Hospital, Boston, Massachusetts, United States of America, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America, Howard Hughes Medical Institute, Boston, Massachusetts, United States of America
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Matthew K. Waldor

* E-mail: mwaldor@rics.bwh.harvard.edu

Affiliations Division of Infectious Diseases, Brigham and Women’s Hospital, Boston, Massachusetts, United States of America, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America, Howard Hughes Medical Institute, Boston, Massachusetts, United States of America
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Vibrio cholerae
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Chemotaxis
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Luciferase
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Luminescence
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Protein-protein interactions
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Protein interactions
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Plasmid construction
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Polymerase chain reaction
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