Avoiding Pitfalls of Internal Controls: Validation of Reference Genes for Analysis by qRT-PCR and Western Blot throughout Rat Retinal Development

Open Access

Peer-reviewed

Research Article

Maurício Rocha-Martins,

Affiliation Neurobiology Department, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
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Brian Njaine,

Affiliation Cell Therapy and Bioengeneering Department, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
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Mariana S. Silveira

* E-mail: silveira@biof.ufrj.br

Affiliation Neurobiology Department, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
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cDNA synthesis
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Protein expression
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