Single-Cell Pulsed-Field Gel Electrophoresis to Detect the Early Stage of DNA Fragmentation in Human Sperm Nuclei

Open Access

Peer-reviewed

Research Article

Satoru Kaneko ,

* E-mail: kaneko@tdc.ac.jp

Affiliation Reproduction Center, Gynecology, Ichikawa General Hospital, Tokyo Dental College, Ichikawa, Chiba, Japan
⨯
Joji Yoshida,

Affiliation Reproduction Center, Gynecology, Ichikawa General Hospital, Tokyo Dental College, Ichikawa, Chiba, Japan
⨯
Hiromichi Ishikawa,

Affiliation Reproduction Center, Urology, Ichikawa General Hospital, Tokyo Dental College, Ichikawa, Chiba, Japan
⨯
Kiyoshi Takamatsu

Affiliation Reproduction Center, Gynecology, Ichikawa General Hospital, Tokyo Dental College, Ichikawa, Chiba, Japan
⨯

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Sperm
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DNA fragmentation
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Serine proteases
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DNA electrophoresis
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DNA damage
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DNA repair
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Deoxyribonucleases
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Pulsed-field gel electrophoresis
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