Recombinant Expression and Purification of T4 Phage Hoc, Soc, gp23, gp24 Proteins in Native Conformations with Stability Studies

Paulina Miernikiewicz; Barbara Owczarek; Agnieszka Piotrowicz; Barbara Boczkowska; Kamila Rzewucka; Grzegorz Figura; Andrey Letarov; Eugene Kulikov; Agnieszka Kopciuch; Kinga Świtała-Jeleń; Anna Oślizło; Katarzyna Hodyra; Jerzy Gubernator; Krystyna Dąbrowska

doi:10.1371/journal.pone.0038902

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Open Access

Peer-reviewed

Research Article

Recombinant Expression and Purification of T4 Phage Hoc, Soc, gp23, gp24 Proteins in Native Conformations with Stability Studies

Paulina Miernikiewicz,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Barbara Owczarek,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Agnieszka Piotrowicz,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Barbara Boczkowska,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Kamila Rzewucka,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Grzegorz Figura,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Andrey Letarov,

Affiliation Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia
⨯
Eugene Kulikov,

Affiliation Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia
⨯
Agnieszka Kopciuch,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Kinga Świtała-Jeleń,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Anna Oślizło,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Katarzyna Hodyra,

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯
Jerzy Gubernator,

Affiliation Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland
⨯
Krystyna Dąbrowska

* E-mail: dabrok@iitd.pan.wroc.pl

Affiliation Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
⨯

Recombinant Expression and Purification of T4 Phage Hoc, Soc, gp23, gp24 Proteins in Native Conformations with Stability Studies

Paulina Miernikiewicz,
Barbara Owczarek,
Agnieszka Piotrowicz,
Barbara Boczkowska,
Kamila Rzewucka,
Grzegorz Figura,
Andrey Letarov,
Eugene Kulikov,
Agnieszka Kopciuch,
Kinga Świtała-Jeleń

Published: July 13, 2012
https://doi.org/10.1371/journal.pone.0038902

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Recombinant proteins
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Protein structure
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Protein denaturation
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Protein structure prediction
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Protein expression
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Phage display
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