Fluorescence Polarization and Fluctuation Analysis Monitors Subunit Proximity, Stoichiometry, and Protein Complex Hydrodynamics

Open Access

Peer-reviewed

Research Article

Tuan A. Nguyen,

Affiliation Section on Cellular Biophotonics, Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland, United States of America
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Pabak Sarkar,

Affiliation Section on Cellular Biophotonics, Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland, United States of America
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Jithesh V. Veetil,

Affiliation Section on Cellular Biophotonics, Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland, United States of America
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Srinagesh V. Koushik,

Affiliation Section on Cellular Biophotonics, Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland, United States of America
⨯
Steven S. Vogel

* E-mail: stevevog@mail.nih.gov

Affiliation Section on Cellular Biophotonics, Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland, United States of America
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Fluorescence resonance energy transfer
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Monomers
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Reporter gene assay
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Transfection
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Anisotropy
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Photons
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Protein complexes
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Protein structure
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