High-Throughput Construction of Intron-Containing Hairpin RNA Vectors for RNAi in Plants

Open Access

Peer-reviewed

Research Article

Pu Yan,

Affiliations South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, China, Graduate University of Chinese Academy of Sciences, Beijing, China
⨯
Wentao Shen,

Affiliation Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, China
⨯
XinZheng Gao,

Affiliation Department of Basic Medical Science, Hainan Medical College, Haikou, China
⨯
Xiaoying Li,

Affiliation Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, China
⨯
Peng Zhou ,

* E-mail: zhp6301@126.com (PZ); duanj@scib.ac.cn (JD)

Affiliation Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, China
⨯
Jun Duan

* E-mail: zhp6301@126.com (PZ); duanj@scib.ac.cn (JD)

Affiliation South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
⨯

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Introns
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Polymerase chain reaction
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Cloning
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RNA interference
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Vector construction
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Vector cloning
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Agrobacteria
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Nicotiana
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