High-Throughput Sequencing of 16S rRNA Gene Amplicons: Effects of Extraction Procedure, Primer Length and Annealing Temperature

Open Access

Peer-reviewed

Research Article

Martin J. Sergeant,

Affiliation Centre for Systems Biology, School of Biosciences, University of Birmingham, Birmingham, United Kingdom
⨯
Chrystala Constantinidou,

Affiliation Centre for Systems Biology, School of Biosciences, University of Birmingham, Birmingham, United Kingdom
⨯
Tristan Cogan,

Affiliation Mucosal Microbiology, School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom
⨯
Charles W. Penn,

Affiliation Centre for Systems Biology, School of Biosciences, University of Birmingham, Birmingham, United Kingdom
⨯
Mark J. Pallen

* E-mail: m.pallen@bham.ac.uk

Affiliation Centre for Systems Biology, School of Biosciences, University of Birmingham, Birmingham, United Kingdom
⨯

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Clostridium
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Ruminococcus
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Ribosomal RNA
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Genetic annealing
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Polymerase chain reaction
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Bifidobacterium
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Campylobacter
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Chickens
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