A Simple and Accurate Two-Step Long DNA Sequences Synthesis Strategy to Improve Heterologous Gene Expression in Pichia

Open Access

Peer-reviewed

Research Article

Jiang-Ke Yang ,

* E-mail: jiangke.yang@gmail.com

Affiliation School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
⨯
Fang-Yuan Chen,

Affiliation School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
⨯
Xiang-Xiang Yan,

Affiliation School of Life Science, Huazhong University of Science and Technology, Wuhan, China
⨯
Li-Hong Miao,

Affiliation School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
⨯
Jiang-Hong Dai

Affiliation School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
⨯

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Polymerase chain reaction
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Oligonucleotides
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DNA synthesis
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Gene expression
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Oligonucleotide synthesis
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Recombinant proteins
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Chemical synthesis
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Fermentation
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