Preparations of Meiotic Pachytene Chromosomes and Extended DNA Fibers from Cotton Suitable for Fluorescence In Situ Hybridization

Renhai Peng; Tao Zhang; Fang Liu; Jian Ling; Chunying Wang; Shaohui Li; Xiangdi Zhang; Yuhong Wang; Kunbo Wang

doi:10.1371/journal.pone.0033847

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Open Access

Peer-reviewed

Research Article

Preparations of Meiotic Pachytene Chromosomes and Extended DNA Fibers from Cotton Suitable for Fluorescence In Situ Hybridization

Renhai Peng ,

Contributed equally to this work with: Renhai Peng, Tao Zhang

Affiliations State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China, Anyang Institute of Technology, Anyang, China
⨯
Tao Zhang ,

Contributed equally to this work with: Renhai Peng, Tao Zhang

Affiliations State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China, Cash Crop Research Institute, Hubei Academy of Agricultural Sciences, Wuhan, China
⨯
Fang Liu,

Affiliation State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China
⨯
Jian Ling,

Affiliation State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China
⨯
Chunying Wang,

Affiliation State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China
⨯
Shaohui Li,

Affiliation State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China
⨯
Xiangdi Zhang,

Affiliation State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China
⨯
Yuhong Wang,

Affiliation State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China
⨯
Kunbo Wang

* E-mail: wkbcri@cricaas.com.cn

Affiliation State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science, Anyang, Henan, China
⨯

Preparations of Meiotic Pachytene Chromosomes and Extended DNA Fibers from Cotton Suitable for Fluorescence In Situ Hybridization

Renhai Peng,
Tao Zhang,
Fang Liu,
Jian Ling,
Chunying Wang,
Shaohui Li,
Xiangdi Zhang,
Yuhong Wang,
Kunbo Wang

Published: March 19, 2012
https://doi.org/10.1371/journal.pone.0033847

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Cotton
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Fluorescent in situ hybridization
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