Ligation Tunes Protein Reactivity in an Ancient Haemoglobin: Kinetic Evidence for an Allosteric Mechanism in Methanosarcina acetivorans Protoglobin

Stefania Abbruzzetti; Lesley Tilleman; Stefano Bruno; Cristiano Viappiani; Filip Desmet; Sabine Van Doorslaer; Massimo Coletta; Chiara Ciaccio; Paolo Ascenzi; Marco Nardini; Martino Bolognesi; Luc Moens; Sylvia Dewilde

doi:10.1371/journal.pone.0033614

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Peer-reviewed

Research Article

Ligation Tunes Protein Reactivity in an Ancient Haemoglobin: Kinetic Evidence for an Allosteric Mechanism in Methanosarcina acetivorans Protoglobin

Stefania Abbruzzetti,

Affiliations Dipartimento di Fisica, Università degli Studi di Parma, Parma, Italy, NEST, Istituto Nanoscienze-CNR, Pisa, Italy
⨯
Lesley Tilleman,

Affiliation Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
⨯
Stefano Bruno,

Affiliation Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi di Parma, Parma, Italy
⨯
Cristiano Viappiani ,

* E-mail: cristiano.viappiani@fis.unipr.it

Affiliations Dipartimento di Fisica, Università degli Studi di Parma, Parma, Italy, NEST, Istituto Nanoscienze-CNR, Pisa, Italy
⨯
Filip Desmet,

Affiliation Department of Physics, University of Antwerp, Antwerp, Belgium
⨯
Sabine Van Doorslaer,

Affiliation Department of Physics, University of Antwerp, Antwerp, Belgium
⨯
Massimo Coletta,

Affiliations Dipartimento di Scienze Cliniche e Medicina Traslazionale, Università di Roma Tor Vergata, Roma, Italy, Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici, Bari, Italy
⨯
Chiara Ciaccio,

Affiliations Dipartimento di Scienze Cliniche e Medicina Traslazionale, Università di Roma Tor Vergata, Roma, Italy, Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici, Bari, Italy
⨯
Paolo Ascenzi,

Affiliation Dipartimento di Biologia, Università Roma Tre, Roma, Italy
⨯
Marco Nardini,

Affiliation Dipartimento di Scienze Biomolecolari e Biotecnologie and CIMAINA, Università degli Studi di Milano, Italy
⨯
Martino Bolognesi,

Affiliation Dipartimento di Scienze Biomolecolari e Biotecnologie and CIMAINA, Università degli Studi di Milano, Italy
⨯
Luc Moens,

Affiliation Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
⨯
Sylvia Dewilde

Affiliation Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
⨯

Ligation Tunes Protein Reactivity in an Ancient Haemoglobin: Kinetic Evidence for an Allosteric Mechanism in Methanosarcina acetivorans Protoglobin

Stefania Abbruzzetti,
Lesley Tilleman,
Stefano Bruno,
Cristiano Viappiani,
Filip Desmet,
Sabine Van Doorslaer,
Massimo Coletta,
Chiara Ciaccio,
Paolo Ascenzi,
Marco Nardini

Published: March 27, 2012
https://doi.org/10.1371/journal.pone.0033614

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