Rapid, Specific Detection of Alphaviruses from Tissue Cultures Using a Replicon-Defective Reporter Gene Assay

Jiangjiao Li; Wuyang Zhu; Huanqin Wang; Jiandong Li; Quanfu Zhang; Ying He; Jia Li; Juanjuan Fu; Dexin Li; Guodong Liang

doi:10.1371/journal.pone.0033007

Loading metrics

Open Access

Peer-reviewed

Research Article

Rapid, Specific Detection of Alphaviruses from Tissue Cultures Using a Replicon-Defective Reporter Gene Assay

Jiangjiao Li ,

Contributed equally to this work with: Jiangjiao Li, Wuyang Zhu

Affiliation Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing, China
⨯
Wuyang Zhu ,

Contributed equally to this work with: Jiangjiao Li, Wuyang Zhu

Affiliation Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing, China
⨯
Huanqin Wang,

Affiliation Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing, China
⨯
Jiandong Li,

Affiliation State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Department of Viral Hemorrhagic Fever (IVDC, China CDC), Beijing, China
⨯
Quanfu Zhang,

Affiliation State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Department of Viral Hemorrhagic Fever (IVDC, China CDC), Beijing, China
⨯
Ying He,

Affiliation Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing, China
⨯
Jia Li,

Affiliation Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing, China
⨯
Juanjuan Fu,

Affiliation Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing, China
⨯
Dexin Li,

Affiliation State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Department of Viral Hemorrhagic Fever (IVDC, China CDC), Beijing, China
⨯
Guodong Liang

* E-mail: gdliang@hotmail.com

Affiliation Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing, China
⨯

Rapid, Specific Detection of Alphaviruses from Tissue Cultures Using a Replicon-Defective Reporter Gene Assay

Jiangjiao Li,
Wuyang Zhu,
Huanqin Wang,
Jiandong Li,
Quanfu Zhang,
Ying He,
Jia Li,
Juanjuan Fu,
Dexin Li,
Guodong Liang

Published: March 12, 2012
https://doi.org/10.1371/journal.pone.0033007

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Alphaviruses
Is the Subject Area "Alphaviruses" applicable to this article?

Thanks for your feedback.
Transfection
Is the Subject Area "Transfection" applicable to this article?

Thanks for your feedback.
Reporter genes
Is the Subject Area "Reporter genes" applicable to this article?

Thanks for your feedback.
Sindbis virus
Is the Subject Area "Sindbis virus" applicable to this article?

Thanks for your feedback.
RNA viruses
Is the Subject Area "RNA viruses" applicable to this article?

Thanks for your feedback.
Chikungunya virus
Is the Subject Area "Chikungunya virus" applicable to this article?

Thanks for your feedback.
Japanese encephalitis virus
Is the Subject Area "Japanese encephalitis virus" applicable to this article?

Thanks for your feedback.
Viral gene expression
Is the Subject Area "Viral gene expression" applicable to this article?

Thanks for your feedback.