FRET-Based Detection of M1 Muscarinic Acetylcholine Receptor Activation by Orthosteric and Allosteric Agonists

Danijela Markovic; Jonathan Holdich; Suleiman Al-Sabah; Rajendra Mistry; Cornelius Krasel; Martyn P. Mahaut-Smith; R. A. John Challiss

doi:10.1371/journal.pone.0029946

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Open Access

Peer-reviewed

Research Article

FRET-Based Detection of M₁ Muscarinic Acetylcholine Receptor Activation by Orthosteric and Allosteric Agonists

Danijela Markovic,

Current address: Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom

Affiliation Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom
⨯
Jonathan Holdich,

Current address: Department of Pharmacology, University of Cambridge, Cambridge, United Kingdom

Affiliation Department of Physiology, Development & Neuroscience, University of Cambridge, Cambridge, United Kingdom
⨯
Suleiman Al-Sabah,

Current address: Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, Safat, Kuwait

Affiliation School of Pharmacy, University of Reading, Whiteknights, Reading, United Kingdom
⨯
Rajendra Mistry,

Affiliation Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom
⨯
Cornelius Krasel,

Current address: Institut für Pharmakologie & Toxikologie, Marburg, Germany

Affiliation School of Pharmacy, University of Reading, Whiteknights, Reading, United Kingdom
⨯
Martyn P. Mahaut-Smith ,

* E-mail: mpms1@leicester.ac.uk (MPM-S); jc36@leicester.ac.uk (RAJC)

Affiliations Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom, Department of Physiology, Development & Neuroscience, University of Cambridge, Cambridge, United Kingdom
⨯
R. A. John Challiss

* E-mail: mpms1@leicester.ac.uk (MPM-S); jc36@leicester.ac.uk (RAJC)

Affiliation Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom
⨯

FRET-Based Detection of M₁ Muscarinic Acetylcholine Receptor Activation by Orthosteric and Allosteric Agonists

Danijela Markovic,
Jonathan Holdich,
Suleiman Al-Sabah,
Rajendra Mistry,
Cornelius Krasel,
Martyn P. Mahaut-Smith,
R. A. John Challiss

Published: January 17, 2012
https://doi.org/10.1371/journal.pone.0029946

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