Probing the SELEX Process with Next-Generation Sequencing

Tatjana Schütze; Barbara Wilhelm; Nicole Greiner; Hannsjörg Braun; Franziska Peter; Mario Mörl; Volker A. Erdmann; Hans Lehrach; Zoltán Konthur; Marcus Menger; Peter F. Arndt; Jörn Glökler

doi:10.1371/journal.pone.0029604

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Open Access

Peer-reviewed

Research Article

Probing the SELEX Process with Next-Generation Sequencing

Tatjana Schütze,

Affiliations Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany, Institute for Chemistry/Biochemistry, Free University Berlin, Berlin, Germany
⨯
Barbara Wilhelm,

Affiliation Department of Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Berlin, Germany
⨯
Nicole Greiner,

Affiliations Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany, Alacris Theranostics GmbH, Berlin, Germany
⨯
Hannsjörg Braun,

Affiliations Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany, Alacris Theranostics GmbH, Berlin, Germany
⨯
Franziska Peter,

Affiliation Institute of Biochemistry, Universität Leipzig, Leipzig, Germany
⨯
Mario Mörl,

Affiliation Institute of Biochemistry, Universität Leipzig, Leipzig, Germany
⨯
Volker A. Erdmann,

Affiliation Institute for Chemistry/Biochemistry, Free University Berlin, Berlin, Germany
⨯
Hans Lehrach,

Affiliation Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
⨯
Zoltán Konthur,

Affiliation Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
⨯
Marcus Menger,

Affiliation RiNA RNA-Netzwerk Technologien GmbH, Berlin, Germany
⨯
Peter F. Arndt,

Affiliation Department of Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Berlin, Germany
⨯
Jörn Glökler

* E-mail: j.gloekler@alacris.de

Affiliations Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany, Alacris Theranostics GmbH, Berlin, Germany
⨯

Probing the SELEX Process with Next-Generation Sequencing

Tatjana Schütze,
Barbara Wilhelm,
Nicole Greiner,
Hannsjörg Braun,
Franziska Peter,
Mario Mörl,
Volker A. Erdmann,
Hans Lehrach,
Zoltán Konthur,
Marcus Menger

Published: December 29, 2011
https://doi.org/10.1371/journal.pone.0029604

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Cloning
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Polymerase chain reaction
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Oligonucleotides
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Binding analysis
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Next-generation sequencing
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DNA denaturation
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DNA libraries
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Dideoxy DNA sequencing
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