A Novel ‘Gene Insertion/Marker Out’ (GIMO) Method for Transgene Expression and Gene Complementation in Rodent Malaria Parasites

Jing-wen Lin; Takeshi Annoura; Mohammed Sajid; Séverine Chevalley-Maurel; Jai Ramesar; Onny Klop; Blandine M. D. Franke-Fayard; Chris J. Janse; Shahid M. Khan

doi:10.1371/journal.pone.0029289

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Open Access

Peer-reviewed

Research Article

A Novel ‘Gene Insertion/Marker Out’ (GIMO) Method for Transgene Expression and Gene Complementation in Rodent Malaria Parasites

Jing-wen Lin ,

Contributed equally to this work with: Jing-wen Lin, Takeshi Annoura

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯
Takeshi Annoura ,

Contributed equally to this work with: Jing-wen Lin, Takeshi Annoura

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯
Mohammed Sajid,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯
Séverine Chevalley-Maurel,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯
Jai Ramesar,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯
Onny Klop,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯
Blandine M. D. Franke-Fayard,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯
Chris J. Janse,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯
Shahid M. Khan

* E-mail: s.m.khan@lumc.nl

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
⨯

A Novel ‘Gene Insertion/Marker Out’ (GIMO) Method for Transgene Expression and Gene Complementation in Rodent Malaria Parasites

Jing-wen Lin,
Takeshi Annoura,
Mohammed Sajid,
Séverine Chevalley-Maurel,
Jai Ramesar,
Onny Klop,
Blandine M. D. Franke-Fayard,
Chris J. Janse,
Shahid M. Khan

Published: December 27, 2011
https://doi.org/10.1371/journal.pone.0029289

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