Protoplast Transformation of Recalcitrant Alkaliphilic Bacillus sp. with Methylated Plasmid DNA and a Developed Hard Agar Regeneration Medium

Open Access

Peer-reviewed

Research Article

Chenghua Gao,

Affiliations State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, Graduate School of the Chinese Academy of Sciences, Beijing, China
⨯
Yanfen Xue,

Affiliation State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
⨯
Yanhe Ma

* E-mail: mayanhe@im.ac.cn

Affiliation State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
⨯

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Bacillus
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Methylation
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Plasmid construction
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DNA methylation
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Bacillus subtilis
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DNA extraction
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Gelatin media
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Genetic transformation
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