Generation and Characterization of Rat and Mouse Monoclonal Antibodies Specific for MeCP2 and Their Use in X-Inactivation Studies

K. Laurence Jost; Andrea Rottach; Manuela Milden; Bianca Bertulat; Annette Becker; Patricia Wolf; Juan Sandoval; Paolo Petazzi; Dori Huertas; Manel Esteller; Elisabeth Kremmer; Heinrich Leonhardt; M. Cristina Cardoso

doi:10.1371/journal.pone.0026499

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Open Access

Peer-reviewed

Research Article

Generation and Characterization of Rat and Mouse Monoclonal Antibodies Specific for MeCP2 and Their Use in X-Inactivation Studies

K. Laurence Jost,

Affiliation Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany
⨯
Andrea Rottach ,

Contributed equally to this work with: Andrea Rottach, Manuela Milden, Bianca Bertulat, Annette Becker, Patricia Wolf

Affiliation Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany
⨯
Manuela Milden ,

Contributed equally to this work with: Andrea Rottach, Manuela Milden, Bianca Bertulat, Annette Becker, Patricia Wolf

Affiliation Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany
⨯
Bianca Bertulat ,

Contributed equally to this work with: Andrea Rottach, Manuela Milden, Bianca Bertulat, Annette Becker, Patricia Wolf

Affiliation Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany
⨯
Annette Becker ,

Contributed equally to this work with: Andrea Rottach, Manuela Milden, Bianca Bertulat, Annette Becker, Patricia Wolf

Affiliation Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany
⨯
Patricia Wolf ,

Contributed equally to this work with: Andrea Rottach, Manuela Milden, Bianca Bertulat, Annette Becker, Patricia Wolf

Affiliation Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany
⨯
Juan Sandoval,

Affiliation Cancer Epigenetics and Biology Program, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain
⨯
Paolo Petazzi,

Affiliation Cancer Epigenetics and Biology Program, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain
⨯
Dori Huertas,

Affiliation Cancer Epigenetics and Biology Program, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain
⨯
Manel Esteller,

Affiliation Cancer Epigenetics and Biology Program, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain
⨯
Elisabeth Kremmer,

Affiliation Helmholtz Center Munich, German Research Center for Environmental Health, Institute of Molecular Immunology, Munich, Germany
⨯
Heinrich Leonhardt,

Affiliation Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany
⨯
M. Cristina Cardoso

* E-mail: Cardoso@bio.tu-darmstadt.de

Affiliation Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany
⨯

Generation and Characterization of Rat and Mouse Monoclonal Antibodies Specific for MeCP2 and Their Use in X-Inactivation Studies

K. Laurence Jost,
Andrea Rottach,
Manuela Milden,
Bianca Bertulat,
Annette Becker,
Patricia Wolf,
Juan Sandoval,
Paolo Petazzi,
Dori Huertas,
Manel Esteller

Published: November 28, 2011
https://doi.org/10.1371/journal.pone.0026499

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05 Jun 2012
18:57 GMT
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Updated Information Relating to This Submission Posted by PLOS_ONE_Group on 05 Jun 2012 at 18:57 GMT

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Immunoprecipitation
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Monoclonal antibodies
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X chromosome inactivation
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DAPI staining
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Antibodies
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Enzyme-linked immunoassays
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Transfection
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Chromatin immunoprecipitation
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