A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq

Open Access

Peer-reviewed

Research Article

Lin Wang,

Affiliation Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York, United States of America
⨯
Yaqing Si,

Affiliation Department of Statistics, Iowa State University, Ames, Iowa, United States of America
⨯
Lauren K. Dedow,

Affiliation Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York, United States of America
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Ying Shao,

Affiliation Department of Microbiology and Immunology, Weill Cornell Medical College, New York, New York, United States of America
⨯
Peng Liu,

Affiliation Department of Statistics, Iowa State University, Ames, Iowa, United States of America
⨯
Thomas P. Brutnell

* E-mail: tpb8@cornell.edu

Affiliation Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York, United States of America
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Gene expression
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Introns
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Sequence alignment
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RNA sequencing
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cDNA libraries
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Polymerase chain reaction
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Genomics
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