A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis

Wentao Xu; Zhifang Zhai; Kunlun Huang; Nan Zhang; Yanfang Yuan; Ying Shang; Yunbo Luo

doi:10.1371/journal.pone.0022900

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Open Access

Peer-reviewed

Research Article

A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis

Wentao Xu ,

Contributed equally to this work with: Wentao Xu, Zhifang Zhai

Affiliations Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China, The Supervision, Inspection and Testing Center of Genetically Modified Food Safety, Ministry of Agriculture, Beijing, China
⨯
Zhifang Zhai ,

Contributed equally to this work with: Wentao Xu, Zhifang Zhai

Affiliation Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
⨯
Kunlun Huang ,

* E-mail: caufoodsafety@gmail.com (KH); lyb@yahoo.cn (YL)

Affiliations Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China, The Supervision, Inspection and Testing Center of Genetically Modified Food Safety, Ministry of Agriculture, Beijing, China
⨯
Nan Zhang,

Affiliation The Supervision, Inspection and Testing Center of Genetically Modified Food Safety, Ministry of Agriculture, Beijing, China
⨯
Yanfang Yuan,

Affiliation Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
⨯
Ying Shang,

Affiliation The Supervision, Inspection and Testing Center of Genetically Modified Food Safety, Ministry of Agriculture, Beijing, China
⨯
Yunbo Luo

* E-mail: caufoodsafety@gmail.com (KH); lyb@yahoo.cn (YL)

Affiliations Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China, The Supervision, Inspection and Testing Center of Genetically Modified Food Safety, Ministry of Agriculture, Beijing, China
⨯

A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis

Wentao Xu,
Zhifang Zhai,
Kunlun Huang,
Nan Zhang,
Yanfang Yuan,
Ying Shang,
Yunbo Luo

Published: January 17, 2012
https://doi.org/10.1371/journal.pone.0022900

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Polymerase chain reaction
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DNA polymerase
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