High-Throughput Identification of Potential Minor Histocompatibility Antigens by MHC Tetramer-Based Screening: Feasibility and Limitations

Open Access

Peer-reviewed

Research Article

Pleun Hombrink ,

* E-mail: p.hombrink@lumc.nl

Affiliation Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Sine R. Hadrup,

Affiliation Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯
Arne Bakker,

Affiliation Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯
Michel G. D. Kester,

Affiliation Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
⨯
J. H. Frederik Falkenburg,

Affiliation Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Peter A. von dem Borne,

Affiliation Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Ton N. M. Schumacher,

Affiliation Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯
Mirjam H. M. Heemskerk

Affiliation Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
⨯

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T cells
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Single nucleotide polymorphisms
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Antigen processing and recognition
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Major histocompatibility complex
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Gene expression
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Cytotoxic T cells
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Binding analysis
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Flow cytometry
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