Efficient Immunoglobulin Gene Disruption and Targeted Replacement in Rabbit Using Zinc Finger Nucleases

Tatiana Flisikowska; Irmgard S. Thorey; Sonja Offner; Francesca Ros; Valeria Lifke; Bryan Zeitler; Oswald Rottmann; Anna Vincent; Lei Zhang; Shirin Jenkins; Helmut Niersbach; Alexander J. Kind; Philip D. Gregory; Angelika E. Schnieke; Josef Platzer

doi:10.1371/journal.pone.0021045

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Open Access

Peer-reviewed

Research Article

Efficient Immunoglobulin Gene Disruption and Targeted Replacement in Rabbit Using Zinc Finger Nucleases

Tatiana Flisikowska ,

Contributed equally to this work with: Tatiana Flisikowska, Irmgard S. Thorey

Affiliation Chair of Livestock Biotechnology, Technische Universität München, Freising, Germany
⨯
Irmgard S. Thorey ,

Contributed equally to this work with: Tatiana Flisikowska, Irmgard S. Thorey

Affiliation Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Sonja Offner,

Affiliation Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Francesca Ros,

Affiliation Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Valeria Lifke,

Affiliation Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Bryan Zeitler,

Affiliation Sangamo BioSciences Inc., Richmond, California, United States of America
⨯
Oswald Rottmann,

Affiliation Chair of Livestock Biotechnology, Technische Universität München, Freising, Germany
⨯
Anna Vincent,

Affiliation Sangamo BioSciences Inc., Richmond, California, United States of America
⨯
Lei Zhang,

Affiliation Sangamo BioSciences Inc., Richmond, California, United States of America
⨯
Shirin Jenkins,

Affiliation Sangamo BioSciences Inc., Richmond, California, United States of America
⨯
Helmut Niersbach,

Affiliation Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Alexander J. Kind,

Affiliation Chair of Livestock Biotechnology, Technische Universität München, Freising, Germany
⨯
Philip D. Gregory,

Affiliation Sangamo BioSciences Inc., Richmond, California, United States of America
⨯
Angelika E. Schnieke ,

* E-mail: schnieke@wzw.tum.de

¶These authors also contributed equally to this work.

Affiliation Chair of Livestock Biotechnology, Technische Universität München, Freising, Germany
⨯
Josef Platzer

¶These authors also contributed equally to this work.

Affiliation Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯

Efficient Immunoglobulin Gene Disruption and Targeted Replacement in Rabbit Using Zinc Finger Nucleases

Tatiana Flisikowska,
Irmgard S. Thorey,
Sonja Offner,
Francesca Ros,
Valeria Lifke,
Bryan Zeitler,
Oswald Rottmann,
Anna Vincent,
Lei Zhang,
Shirin Jenkins

Published: June 13, 2011
https://doi.org/10.1371/journal.pone.0021045

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Rabbits
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Gene targeting
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Oocytes
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Zinc finger nucleases
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