Bicistronic Lentiviruses Containing a Viral 2A Cleavage Sequence Reliably Co-Express Two Proteins and Restore Vision to an Animal Model of LCA1

Jonathan D. Verrier; Irina Madorsky; William E. Coggin; Mero Geesey; Michael Hochman; Elleanor Walling; Daniel Daroszewski; Kristofer S. Eccles; Rachel Ludlow; Susan L. Semple-Rowland

doi:10.1371/journal.pone.0020553

Loading metrics

Open Access

Peer-reviewed

Research Article

Bicistronic Lentiviruses Containing a Viral 2A Cleavage Sequence Reliably Co-Express Two Proteins and Restore Vision to an Animal Model of LCA1

Jonathan D. Verrier,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
Irina Madorsky,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
William E. Coggin,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
Mero Geesey,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
Michael Hochman,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
Elleanor Walling,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
Daniel Daroszewski,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
Kristofer S. Eccles,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
Rachel Ludlow,

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯
Susan L. Semple-Rowland

* E-mail: rowland@mbi.ufl.edu

Affiliation Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, United States of America
⨯

Bicistronic Lentiviruses Containing a Viral 2A Cleavage Sequence Reliably Co-Express Two Proteins and Restore Vision to an Animal Model of LCA1

Jonathan D. Verrier,
Irina Madorsky,
William E. Coggin,
Mero Geesey,
Michael Hochman,
Elleanor Walling,
Daniel Daroszewski,
Kristofer S. Eccles,
Rachel Ludlow,
Susan L. Semple-Rowland

Published: May 27, 2011
https://doi.org/10.1371/journal.pone.0020553

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Photoreceptors
Is the Subject Area "Photoreceptors" applicable to this article?

Thanks for your feedback.
Retina
Is the Subject Area "Retina" applicable to this article?

Thanks for your feedback.
Transfection
Is the Subject Area "Transfection" applicable to this article?

Thanks for your feedback.
Chickens
Is the Subject Area "Chickens" applicable to this article?

Thanks for your feedback.
Vision
Is the Subject Area "Vision" applicable to this article?

Thanks for your feedback.
Gene therapy
Is the Subject Area "Gene therapy" applicable to this article?

Thanks for your feedback.
Protein expression
Is the Subject Area "Protein expression" applicable to this article?

Thanks for your feedback.
Cell cultures
Is the Subject Area "Cell cultures" applicable to this article?

Thanks for your feedback.