Cell-free H-cluster Synthesis and [FeFe] Hydrogenase Activation: All Five CO and CN⁻ Ligands Derive from Tyrosine

• Jon M. Kuchenreuther,

  Affiliation Department of Chemical Engineering, Stanford University, Stanford, California, United States of America

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• Simon J. George,

  Affiliations Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, Department of Applied Science, University of California Davis, Davis, California, United States of America

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• Celestine S. Grady-Smith,

  Affiliation Department of Applied Science, University of California Davis, Davis, California, United States of America

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• Stephen P. Cramer,

  Affiliations Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, Department of Applied Science, University of California Davis, Davis, California, United States of America

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• James R. Swartz

  * E-mail: jswartz@stanford.edu

  Affiliations Department of Chemical Engineering, Stanford University, Stanford, California, United States of America, Department of Bioengineering, Stanford University, Stanford, California, United States of America

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