Multiplex Fluorescence Melting Curve Analysis for Mutation Detection with Dual-Labeled, Self-Quenched Probes

Open Access

Peer-reviewed

Research Article

Qiuying Huang,

Affiliation Engineering Research Center of Molecular Diagnostics, Ministry of Education, Department of Biomedical Sciences, School of Life Sciences, and the Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen, Fujian, China
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Zanzan Liu,

Affiliation Engineering Research Center of Molecular Diagnostics, Ministry of Education, Department of Biomedical Sciences, School of Life Sciences, and the Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen, Fujian, China
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Yiqun Liao,

Affiliation Institute for Biomedical Research, Xiamen University, Xiamen, Fujian, China
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Xiaoyun Chen,

Affiliation Engineering Research Center of Molecular Diagnostics, Ministry of Education, Department of Biomedical Sciences, School of Life Sciences, and the Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen, Fujian, China
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Yi Zhang,

Affiliation Institute for Biomedical Research, Xiamen University, Xiamen, Fujian, China
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Qingge Li

* E-mail: qgli@xmu.edu.cn

Affiliation Engineering Research Center of Molecular Diagnostics, Ministry of Education, Department of Biomedical Sciences, School of Life Sciences, and the Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen, Fujian, China
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Polymerase chain reaction
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Mutation detection
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Melting
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Probe hybridization
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Multiplexing
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Denaturation
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Genotyping
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Oligonucleotides
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