Rapid KRAS, EGFR, BRAF and PIK3CA Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer Using Allele-Specific qPCR

Ronald van Eijk; Jappe Licht; Melanie Schrumpf; Mehrdad Talebian Yazdi; Dina Ruano; Giusi I. Forte; Petra M. Nederlof; Maud Veselic; Klaus F. Rabe; Jouke T. Annema; Vincent Smit; Hans Morreau; Tom van Wezel

doi:10.1371/journal.pone.0017791

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Open Access

Peer-reviewed

Research Article

Rapid KRAS, EGFR, BRAF and PIK3CA Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer Using Allele-Specific qPCR

Ronald van Eijk ,

Contributed equally to this work with: Ronald van Eijk, Jappe Licht

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Jappe Licht ,

Contributed equally to this work with: Ronald van Eijk, Jappe Licht

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Melanie Schrumpf,

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Mehrdad Talebian Yazdi,

Affiliation Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Dina Ruano,

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Giusi I. Forte,

Current address: Department di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Petra M. Nederlof,

Affiliation Department of Pathology, Netherlands Cancer Institute NKI-AVL, Amsterdam, The Netherlands
⨯
Maud Veselic,

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Klaus F. Rabe,

Affiliation Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Jouke T. Annema,

Affiliation Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Vincent Smit,

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Hans Morreau,

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Tom van Wezel

* E-mail: t.van_wezel@lumc.nl

Affiliation Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
⨯

Rapid KRAS, EGFR, BRAF and PIK3CA Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer Using Allele-Specific qPCR

Ronald van Eijk,
Jappe Licht,
Melanie Schrumpf,
Mehrdad Talebian Yazdi,
Dina Ruano,
Giusi I. Forte,
Petra M. Nederlof,
Maud Veselic,
Klaus F. Rabe,
Jouke T. Annema

Published: March 8, 2011
https://doi.org/10.1371/journal.pone.0017791

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Non-small cell lung cancer
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