A New Approach for Heparin Standardization: Combination of Scanning UV Spectroscopy, Nuclear Magnetic Resonance and Principal Component Analysis

Marcelo A. Lima; Timothy R. Rudd; Eduardo H. C. de Farias; Lyvia F. Ebner; Tarsis F. Gesteira; Lauro M. de Souza; Aline Mendes; Carolina R. Córdula; João R. M. Martins; Debra Hoppensteadt; Jawed Fareed; Guilherme L. Sassaki; Edwin A. Yates; Ivarne L. S. Tersariol; Helena B. Nader

doi:10.1371/journal.pone.0015970

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Open Access

Peer-reviewed

Research Article

A New Approach for Heparin Standardization: Combination of Scanning UV Spectroscopy, Nuclear Magnetic Resonance and Principal Component Analysis

Marcelo A. Lima,

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯
Timothy R. Rudd,

Affiliation School of Biological Sciences, University of Liverpool, Liverpool, United Kingdom
⨯
Eduardo H. C. de Farias,

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯
Lyvia F. Ebner,

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯
Tarsis F. Gesteira,

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯
Lauro M. de Souza,

Affiliation Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil
⨯
Aline Mendes,

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯
Carolina R. Córdula,

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯
João R. M. Martins,

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯
Debra Hoppensteadt,

Affiliation Department of Pathology, Loyola University Medical Center, Maywood, Illinois, United States of America
⨯
Jawed Fareed,

Affiliation Department of Pathology, Loyola University Medical Center, Maywood, Illinois, United States of America
⨯
Guilherme L. Sassaki,

Affiliation Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil
⨯
Edwin A. Yates,

Affiliation School of Biological Sciences, University of Liverpool, Liverpool, United Kingdom
⨯
Ivarne L. S. Tersariol,

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯
Helena B. Nader

* E-mail: hbnader.bioq@epm.br

Affiliation Departamento de Bioquímica, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, São Paulo, Brazil
⨯

A New Approach for Heparin Standardization: Combination of Scanning UV Spectroscopy, Nuclear Magnetic Resonance and Principal Component Analysis

Marcelo A. Lima,
Timothy R. Rudd,
Eduardo H. C. de Farias,
Lyvia F. Ebner,
Tarsis F. Gesteira,
Lauro M. de Souza,
Aline Mendes,
Carolina R. Córdula,
João R. M. Martins,
Debra Hoppensteadt

Published: January 18, 2011
https://doi.org/10.1371/journal.pone.0015970

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Heparin
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NMR spectroscopy
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Principal component analysis
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Ultraviolet spectroscopy
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