Isolation of Proteinase K-Sensitive Prions Using Pronase E and Phosphotungstic Acid

Open Access

Peer-reviewed

Research Article

Laura D'Castro,

Affiliation MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, National Hospital for Neurology and Neurosurgery, London, United Kingdom
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Adam Wenborn,

Affiliation MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, National Hospital for Neurology and Neurosurgery, London, United Kingdom
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Nathalie Gros,

Affiliation MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, National Hospital for Neurology and Neurosurgery, London, United Kingdom
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Susan Joiner,

Affiliation MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, National Hospital for Neurology and Neurosurgery, London, United Kingdom
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Sabrina Cronier,

Current address: INRA, U892 Virologie et Immunologie Moleculaires, Jouy-en-Josas, France

Affiliation MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, National Hospital for Neurology and Neurosurgery, London, United Kingdom
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John Collinge,

Affiliation MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, National Hospital for Neurology and Neurosurgery, London, United Kingdom
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Jonathan D. F. Wadsworth

* E-mail: j.d.wadsworth@prion.ucl.ac.uk

Affiliation MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, National Hospital for Neurology and Neurosurgery, London, United Kingdom
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Prions
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Animal prion diseases
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Proteases
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Prion diseases
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Immunoblotting
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Silver staining
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Brain diseases
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Enzyme-linked immunoassays
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