Evaluation of a Quantitative Real-Time PCR Assay to Measure HIV-Specific Mucosal CD8+ T Cell Responses in the Cervix

• Duncan Chege ,

  * E-mail: duncan.chege@utoronto.ca

  Affiliation Department of Medicine, University of Toronto, Toronto, Ontario, Canada

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• Yijie Chai,

  Affiliation Department of Medicine, University of Toronto, Toronto, Ontario, Canada

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• Sanja Huibner,

  Affiliation Department of Medicine, University of Toronto, Toronto, Ontario, Canada

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• Lyle McKinnon,

  Affiliations Department of Medicine, University of Toronto, Toronto, Ontario, Canada, Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada, Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

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• Charles Wachihi,

  Affiliation Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

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• Makubo Kimani,

  Affiliation Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

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• Walter Jaoko,

  Affiliation Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

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• Joshua Kimani,

  Affiliations Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada, Laboratory for HIV Immunology, National HIV and Retrovirology Laboratories, Public Health Agency of Canada, Winnipeg, Canada, Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

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• T. Blake Ball,

  Affiliations Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada, Laboratory for HIV Immunology, National HIV and Retrovirology Laboratories, Public Health Agency of Canada, Winnipeg, Canada, Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

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• Francis A. Plummer,

  Affiliations Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada, Laboratory for HIV Immunology, National HIV and Retrovirology Laboratories, Public Health Agency of Canada, Winnipeg, Canada, Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

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• Rupert Kaul ,

  Contributed equally to this work with: Rupert Kaul, Anuradha Rebbapragada

  Affiliations Department of Medicine, University of Toronto, Toronto, Ontario, Canada, Departments of Medicine, University Health Network, Toronto, Ontario, Canada, Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

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• Anuradha Rebbapragada

  Contributed equally to this work with: Rupert Kaul, Anuradha Rebbapragada

  Affiliations Public Health Laboratory-Toronto, Ontario Agency for Health Protection and Promotion, Toronto, Ontario, Canada, Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

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