Characterization of the Raf Kinase Inhibitory Protein (RKIP) Binding Pocket: NMR-Based Screening Identifies Small-Molecule Ligands

Anne N. Shemon; Gary L. Heil; Alexey E. Granovsky; Mathew M. Clark; Dan McElheny; Alexander Chimon; Marsha R. Rosner; Shohei Koide

doi:10.1371/journal.pone.0010479

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Peer-reviewed

Research Article

Characterization of the Raf Kinase Inhibitory Protein (RKIP) Binding Pocket: NMR-Based Screening Identifies Small-Molecule Ligands

Anne N. Shemon ,

Contributed equally to this work with: Anne N. Shemon, Gary L. Heil

Affiliation Ben May Department for Cancer Research, University of Chicago, Chicago, Illinois, United States of America
⨯
Gary L. Heil ,

Contributed equally to this work with: Anne N. Shemon, Gary L. Heil

Affiliation Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, United States of America
⨯
Alexey E. Granovsky,

Affiliation Ben May Department for Cancer Research, University of Chicago, Chicago, Illinois, United States of America
⨯
Mathew M. Clark,

Affiliations Ben May Department for Cancer Research, University of Chicago, Chicago, Illinois, United States of America, Department of Neurobiology, Pharmacology and Physiology, University of Chicago, Chicago, Illinois, United States of America
⨯
Dan McElheny,

Affiliation Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, United States of America
⨯
Alexander Chimon,

Affiliation Ben May Department for Cancer Research, University of Chicago, Chicago, Illinois, United States of America
⨯
Marsha R. Rosner ,

* E-mail: m-rosner@uchicago.edu

¶These authors also contributed equally to this work.

Affiliations Ben May Department for Cancer Research, University of Chicago, Chicago, Illinois, United States of America, Department of Neurobiology, Pharmacology and Physiology, University of Chicago, Chicago, Illinois, United States of America
⨯
Shohei Koide

¶These authors also contributed equally to this work.

Affiliation Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, United States of America
⨯

Characterization of the Raf Kinase Inhibitory Protein (RKIP) Binding Pocket: NMR-Based Screening Identifies Small-Molecule Ligands

Anne N. Shemon,
Gary L. Heil,
Alexey E. Granovsky,
Mathew M. Clark,
Dan McElheny,
Alexander Chimon,
Marsha R. Rosner,
Shohei Koide

Published: May 5, 2010
https://doi.org/10.1371/journal.pone.0010479

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NMR spectroscopy
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HeLa cells
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Phosphorylation
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Protein kinase signaling cascade
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Binding analysis
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Crystal structure
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