Lactococcus lactis, an Alternative System for Functional Expression of Peripheral and Intrinsic Arabidopsis Membrane Proteins

Annie Frelet-Barrand; Sylvain Boutigny; Lucas Moyet; Aurélien Deniaud; Daphné Seigneurin-Berny; Daniel Salvi; Florent Bernaudat; Pierre Richaud; Eva Pebay-Peyroula; Jacques Joyard; Norbert Rolland

doi:10.1371/journal.pone.0008746

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Research Article

Lactococcus lactis, an Alternative System for Functional Expression of Peripheral and Intrinsic Arabidopsis Membrane Proteins

Annie Frelet-Barrand ,

Contributed equally to this work with: Annie Frelet-Barrand, Sylvain Boutigny

Affiliations CNRS, Laboratoire de Physiologie Cellulaire Végétale, UMR5168, Grenoble, France, CEA, DSV, iRTSV, LPCV, Grenoble, France, INRA, Laboratoire de Physiologie Cellulaire Végétale, UMR1200, Grenoble, France, Université Joseph Fourier, Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France
⨯
Sylvain Boutigny ,

Contributed equally to this work with: Annie Frelet-Barrand, Sylvain Boutigny

Affiliations CNRS, Laboratoire de Physiologie Cellulaire Végétale, UMR5168, Grenoble, France, CEA, DSV, iRTSV, LPCV, Grenoble, France, INRA, Laboratoire de Physiologie Cellulaire Végétale, UMR1200, Grenoble, France, Université Joseph Fourier, Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France
⨯
Lucas Moyet,

Affiliations CNRS, Laboratoire de Physiologie Cellulaire Végétale, UMR5168, Grenoble, France, CEA, DSV, iRTSV, LPCV, Grenoble, France, INRA, Laboratoire de Physiologie Cellulaire Végétale, UMR1200, Grenoble, France, Université Joseph Fourier, Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France
⨯
Aurélien Deniaud,

Affiliations CEA, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France, CNRS, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France, Université Joseph Fourier, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France
⨯
Daphné Seigneurin-Berny ,

* E-mail: daphne.berny@cea.fr

Affiliations CNRS, Laboratoire de Physiologie Cellulaire Végétale, UMR5168, Grenoble, France, CEA, DSV, iRTSV, LPCV, Grenoble, France, INRA, Laboratoire de Physiologie Cellulaire Végétale, UMR1200, Grenoble, France, Université Joseph Fourier, Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France
⨯
Daniel Salvi,

Affiliations CNRS, Laboratoire de Physiologie Cellulaire Végétale, UMR5168, Grenoble, France, CEA, DSV, iRTSV, LPCV, Grenoble, France, INRA, Laboratoire de Physiologie Cellulaire Végétale, UMR1200, Grenoble, France, Université Joseph Fourier, Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France
⨯
Florent Bernaudat,

Affiliations CEA, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France, CNRS, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France, Université Joseph Fourier, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France
⨯
Pierre Richaud,

Affiliations CEA, DSV, iBEB, Laboratoire des Echanges Membranaires et Signalisation, St Paul les Durance, France, CNRS, UMR 6191, St Paul les Durance, France, Université Aix-Marseille, St Paul les Durance, France
⨯
Eva Pebay-Peyroula,

Affiliations CEA, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France, CNRS, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France, Université Joseph Fourier, IBS Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France
⨯
Jacques Joyard,

Affiliations CNRS, Laboratoire de Physiologie Cellulaire Végétale, UMR5168, Grenoble, France, CEA, DSV, iRTSV, LPCV, Grenoble, France, INRA, Laboratoire de Physiologie Cellulaire Végétale, UMR1200, Grenoble, France, Université Joseph Fourier, Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France
⨯
Norbert Rolland

Affiliations CNRS, Laboratoire de Physiologie Cellulaire Végétale, UMR5168, Grenoble, France, CEA, DSV, iRTSV, LPCV, Grenoble, France, INRA, Laboratoire de Physiologie Cellulaire Végétale, UMR1200, Grenoble, France, Université Joseph Fourier, Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France
⨯

Lactococcus lactis, an Alternative System for Functional Expression of Peripheral and Intrinsic Arabidopsis Membrane Proteins

Annie Frelet-Barrand,
Sylvain Boutigny,
Lucas Moyet,
Aurélien Deniaud,
Daphné Seigneurin-Berny,
Daniel Salvi,
Florent Bernaudat,
Pierre Richaud,
Eva Pebay-Peyroula,
Jacques Joyard

Published: January 20, 2010
https://doi.org/10.1371/journal.pone.0008746

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Membrane proteins
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Lactococcus lactis
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Recombinant proteins
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Arabidopsis thaliana
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Chloroplasts
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Cloning
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Protein expression
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