Kinetic Characterization and Phosphoregulation of the Francisella tularensis 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase (MEP Synthase)

Safdar Jawaid; Heather Seidle; Weidong Zhou; Hafsa Abdirahman; Maher Abadeer; Joseph H. Hix; Monique L. van Hoek; Robin D. Couch

doi:10.1371/journal.pone.0008288

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Research Article

Kinetic Characterization and Phosphoregulation of the Francisella tularensis 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase (MEP Synthase)

Safdar Jawaid,

Affiliations Department of Chemistry and Biochemistry, George Mason University, Manassas, Virginia, United States of America, National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, United States of America
⨯
Heather Seidle,

Affiliations Department of Chemistry and Biochemistry, George Mason University, Manassas, Virginia, United States of America, National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, United States of America
⨯
Weidong Zhou,

Affiliations Department of Molecular and Microbiology, George Mason University, Manassas, Virginia, United States of America, Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia, United States of America
⨯
Hafsa Abdirahman,

Affiliations Department of Chemistry and Biochemistry, George Mason University, Manassas, Virginia, United States of America, National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, United States of America
⨯
Maher Abadeer,

Affiliations Department of Chemistry and Biochemistry, George Mason University, Manassas, Virginia, United States of America, National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, United States of America
⨯
Joseph H. Hix,

Affiliations Department of Molecular and Microbiology, George Mason University, Manassas, Virginia, United States of America, National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, United States of America
⨯
Monique L. van Hoek,

Affiliations Department of Molecular and Microbiology, George Mason University, Manassas, Virginia, United States of America, National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, United States of America
⨯
Robin D. Couch

* E-mail: rcouch@gmu.edu

Affiliations Department of Chemistry and Biochemistry, George Mason University, Manassas, Virginia, United States of America, National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, United States of America
⨯

Kinetic Characterization and Phosphoregulation of the Francisella tularensis 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase (MEP Synthase)

Safdar Jawaid,
Heather Seidle,
Weidong Zhou,
Hafsa Abdirahman,
Maher Abadeer,
Joseph H. Hix,
Monique L. van Hoek,
Robin D. Couch

Published: December 14, 2009
https://doi.org/10.1371/journal.pone.0008288

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Francisella tularensis
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Polymerase chain reaction
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Phosphorylation
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Enzyme assays
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Cloning
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Protein expression
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