Transposition-Based Method for the Rapid Generation of Gene-Targeting Vectors to Produce Cre/Flp-Modifiable Conditional Knock-Out Mice

Open Access

Peer-reviewed

Research Article

Hilkka Turakainen,

Affiliation Program in Cellular Biotechnology, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Helsinki, Finland
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Jonna Saarimäki-Vire,

Affiliation Program in Developmental Biology, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Helsinki, Finland
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Natalia Sinjushina,

Affiliation Program in Developmental Biology, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Helsinki, Finland
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Juha Partanen,

Affiliation Program in Developmental Biology, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Helsinki, Finland
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Harri Savilahti

* E-mail: harri.savilahti@utu.fi

Affiliations Program in Cellular Biotechnology, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Helsinki, Finland, Division of Genetics and Physiology, Department of Biology, University of Turku, Turku, Finland
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Transposable elements
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Gene targeting
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Polymerase chain reaction
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Cloning
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Vector cloning
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Plasmid construction
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Gene pool
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Vector construction
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