BRED: A Simple and Powerful Tool for Constructing Mutant and Recombinant Bacteriophage Genomes

Open Access

Peer-reviewed

Research Article

Laura J. Marinelli,

Affiliation Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯
Mariana Piuri,

Affiliation Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯
Zuzana Swigoňová,

Affiliation Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
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Amrita Balachandran,

Affiliation Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
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Lauren M. Oldfield,

Affiliation Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
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Julia C. van Kessel,

Affiliation Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯
Graham F. Hatfull

* E-mail: gfh@pitt.edu

Affiliation Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯

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Bacteriophages
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Polymerase chain reaction
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DNA recombination
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Mycobacteria
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Homologous recombination
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Genomics
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Mutagenesis
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Viral genomics
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