Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity

• Bryan J. Jones,

  Roles Conceptualization, Investigation, Methodology, Writing – original draft, Writing – review & editing

  Current address: Bio-Techne Corporation, Minneapolis, Minnesota, United States of America

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  http://orcid.org/0000-0002-9863-7119

  ⨯
• Robert L. Evans III,

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  http://orcid.org/0000-0002-1690-9927

  ⨯
• Nathan J. Mylrea,

  Roles Formal analysis, Investigation, Writing – original draft, Writing – review & editing

  Current address: Pace Analytical Services, LLC, Minneapolis, Minnesota, United States of America

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  ⨯
• Debayan Chaudhury,

  Roles Investigation, Methodology, Writing – review & editing

  Current address: Department of Biochemistry, University of Wisconsin, Madison, Wisconsin, United States of America

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  ⨯
• Christine Luo,

  Roles Data curation, Formal analysis, Investigation, Visualization, Writing – original draft

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  ⨯
• Bo Guan,

  Roles Investigation, Supervision, Writing – review & editing

  Current address: School of Food Science, Shihezi University, Shihezi, Xinjiang, China

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  http://orcid.org/0000-0001-7014-9941

  ⨯
• Colin T. Pierce,

  Roles Investigation, Writing – review & editing

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  http://orcid.org/0000-0001-9506-9753

  ⨯
• Wendy R. Gordon,

  Roles Funding acquisition, Supervision, Writing – review & editing

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  http://orcid.org/0000-0001-7696-5560

  ⨯
• Carrie M. Wilmot,

  Roles Funding acquisition, Resources, Writing – review & editing

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  http://orcid.org/0000-0002-8706-0000

  ⨯
• Romas J. Kazlauskas

  Roles Conceptualization, Funding acquisition, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: rjk@umn.edu

  Affiliation Department of Biochemistry, Molecular Biology and Biophysics and The Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota, United States of America

  http://orcid.org/0000-0002-3570-2411

  ⨯